Sds Page Technique (1)

SDS-PAGE Technique SDS-PAGE, which full name is sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis, is a powerful tool for protein analysis (molecular masses between 5 and 250 kDa) due to its availability and reproducibility. The combination of... More

SDS-PAGE Technique SDS-PAGE, which full name is sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis, is a powerful tool for protein analysis (molecular masses between 5 and 250 kDa) due to its availability and reproducibility. The combination of SDS and polyacrylamide gel allows this technique to eliminate the influence of proteins' structure and charge, and achieve separation only based on differences in their molecular weight. Moreover, SDS-PAGE can also be used for purity characterization of PEGylated proteins after purification steps and quantification of PEGylation extent by studying the molecular weight of appeared bands on gel. Sodium dodecyl sulfate, namely SDS, is an anionic detergent which can break the intra- and intermolecular hydrogen bonds, further destroying the secondary and tertiary structure of protein molecules. Moreover, strong reducing agents, such as mercaptoethanol and dithiothreitol, can break the disulfide bond between cysteine residues. In SDS-PAGE Less